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(Plasmid #159613)


Item Catalog # Description Quantity Price (USD)
Plasmid 159613 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Addgene Plasmid #26103
  • Backbone size w/o insert (bp) 5356
  • Total vector size (bp) 7387
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Covid-SARS2 Nsp14
  • Species
    Synthetic; COVID-SARS2
  • Insert Size (bp)
  • Mutation
    Codon-optimized for E. coli expression
  • GenBank ID
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter T7 - LacO
  • Tag / Fusion Protein
    • His6, TEV cleavage site (N terminal on backbone)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Covid-SARS2 Nsp10
  • Species
    Synthetic; COVID-SARS2
  • Insert Size (bp)
  • Mutation
    Codon-optimized for E. coli expression
  • GenBank ID
  • Promoter (Bicistronic)
  • Tag / Fusion Protein
    • none

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    COVID-SARS2 NSP14/10 was a gift from Opher Gileadi (Addgene plasmid # 159613 ; ; RRID:Addgene_159613)
  • For your References section:

    Characterization of the SARS-CoV-2 ExoN (nsp14ExoN-nsp10) complex: implications for its role in viral genome stability and inhibitor identification. Baddock HT, Brolih S, Yosaatmadja Y, Ratnaweera M, Bielinski M, Swift LP, Cruz-Migoni A, Fan H, Keown JR, Walker AP, Morris GM, Grimes JM, Fodor E, Schofield CJ, Gileadi O, McHugh PJ. Nucleic Acids Res. 2022 Jan 17. pii: 6509090. doi: 10.1093/nar/gkab1303. 10.1093/nar/gkab1303 PubMed 35037045