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pET28a-SARS-CoV-2 NSP13
(Plasmid #176492)


Item Catalog # Description Quantity Price (USD)
Plasmid 176492 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

* Login to view industry pricing.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 7147
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    SARS-CoV-2 NSP13
  • Species
  • Mutation
    I570 from SARS-CoV NSP13 was mutated to V to convert it to SARS-CoV-2 NSP13
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter T7
  • Tag / Fusion Protein
    • His6 and T7 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We received the clone for SARS-CoV NSP13 from Dr. Jiandong Huang from University of Hong Kong

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The original vector pHelA12 was described in a paper published by Dr. Huang (PMID: 12917423). We mutated the I570 of the original SARS-CoV NSP13 to V to convert it to SARS-CoV-2 NSP13.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-SARS-CoV-2 NSP13 was a gift from Xiaodong Cheng (Addgene plasmid # 176492 ; ; RRID:Addgene_176492)
  • For your References section:

    Discovery of COVID-19 Inhibitors Targeting the SARS-CoV-2 Nsp13 Helicase. White MA, Lin W, Cheng X. J Phys Chem Lett. 2020 Nov 5;11(21):9144-9151. doi: 10.1021/acs.jpclett.0c02421. Epub 2020 Oct 14. 10.1021/acs.jpclett.0c02421 PubMed 33052685