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pEF6-Lck-TurboID
(Plasmid #159433)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159433 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 159433-D50 50 μg of DNA in Tris buffer $495
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DNA 159433-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEF6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5818
  • Total vector size (bp) 8249
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Lck
  • Alt name
    Lck tyrosine kinase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1527
  • GenBank ID
    NM_001042771.2
  • Entrez Gene
    LCK (a.k.a. IMD22, LSK, YT16, p56lck, pp58lck)
  • Promoter EF-1a

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BbvCI (unknown if destroyed)
  • 5′ sequencing primer TATATGAATTCGCCATGGGCTGTGGCTGC
  • 3′ sequencing primer ATATGTTTAAACTCAAGCGTAATCTGGAACATCGTATGGGTAAGGCTGAGGCTGGTACTG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    TurboID (BirA mutant)
  • Species
    E. coli
  • Insert Size (bp)
    969
  • Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, M241T, S263P, I305V
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbvCI (unknown if destroyed)
  • 3′ cloning site PmeI (unknown if destroyed)
  • 5′ sequencing primer ATATGTTTAAACTCAAGCGTAATCTGGAACATCGTATGGGTAAGGCTGAGGCTGGTACTG
  • 3′ sequencing primer TATATGTTTAAACTTAAGCGTAATCTGGAACATCGTATGGGTATCCGTCCAGGGTCAGGCGCTCC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    TurboID obtained from Addgene #107169 (Alice Ting Lab)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF6-Lck-TurboID was a gift from Arthur Salomon (Addgene plasmid # 159433 ; http://n2t.net/addgene:159433 ; RRID:Addgene_159433)

  • For your References section:

    Quantitative Interactomics of Lck-TurboID in Living Human T Cells Unveils T Cell Receptor Stimulation-Induced Proximal Lck Interactors. Chua XY, Aballo T, Elnemer W, Tran M, Salomon A. J Proteome Res. 2020 Nov 13. doi: 10.1021/acs.jproteome.0c00616. 10.1021/acs.jproteome.0c00616 PubMed 33185455
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