Skip to main content
Addgene

FLX1.8NFATGFPpd2HS4
(Plasmid #169096)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169096 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    FL1.8WPREHS4
  • Total vector size (bp) 6717
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    NFAT enhancer (from IL-4 promoter)
  • Species
    H. sapiens (human)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nsi I (not destroyed)
  • 3′ cloning site Bgl II (not destroyed)
  • 5′ sequencing primer -
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Destabilized green fluorescent protein
  • Alt name
    EGFPpd2
  • Species
    Aequorea victoria
  • Insert Size (bp)
    855
  • Promoter NFAT enhancer + synthetic TATA box

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Mlu I (not destroyed)
  • 3′ cloning site Sal I (not destroyed)
  • 5′ sequencing primer -
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    NFAT ehancer was PCR amplified from 9X NFAT-apha-MHC-Luc (Addgene plasmid 5194)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLX1.8NFATGFPpd2HS4 was a gift from Geoffrey Owens (Addgene plasmid # 169096 ; http://n2t.net/addgene:169096 ; RRID:Addgene_169096)