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8xNFAT-ZsG-hCD8
(Plasmid #153417)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 153417 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSIR
  • Backbone manufacturer
    Maki Nakayama
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene
  • Species
    Other
  • Insert Size (bp)
    998
  • Entrez Gene
    Cd8a (a.k.a. BB154331, Ly-2, Ly-35, Ly-B, Lyt-2)

Cloning Information

Resource Information

Depositor Comments

Second gene is human CD8

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8xNFAT-ZsG-hCD8 was a gift from Maki Nakayama (Addgene plasmid # 153417 ; http://n2t.net/addgene:153417 ; RRID:Addgene_153417)
  • For your References section:

    Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation Reporter-Based Detection System. Mann SE, Zhou Z, Landry LG, Anderson AM, Alkanani AK, Fischer J, Peakman M, Mallone R, Campbell K, Michels AW, Nakayama M. Front Immunol. 2020 Apr 9;11:633. doi: 10.3389/fimmu.2020.00633. eCollection 2020. 10.3389/fimmu.2020.00633 PubMed 32328071