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Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation Reporter-Based Detection System.
Mann SE, Zhou Z, Landry LG, Anderson AM, Alkanani AK, Fischer J, Peakman M, Mallone R, Campbell K, Michels AW, Nakayama M
Front Immunol. 2020 Apr 9;11:633. doi: 10.3389/fimmu.2020.00633. eCollection 2020.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
153415A2_uSFFVExpresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter
1534178xNFAT-ZsG-hCD8NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene
153418CD3-2A_pMI-LORetroviral (MSCV) expression of all four WT CD3 subunits, co-expressed with LSSmOrange
153423pMIII-Neo-mCheRetroviral (MSCV) expression of mCherry fluorescent protein
153424pMIII-Neo-tdTomatoRetroviral (MSCV) expression of tdTomato fluorescent protein
153425pMIII-Neo-E2CrimsonRetroviral (MSCV) expression of E2-Crimson fluorescent protein
153426pMIII-Neo-BFPRetroviral (MSCV) expression of mTagBFP2 fluorescent protein
153427pMIII-Neo-AMRetroviral (MSCV) expression of ametrine fluorescent protein
153428pMIII-Neo-LORetroviral (MSCV) expression of LSSmOrange fluorescent protein

Antibodies from Article