pMIII-Neo-tdTomato
(Plasmid #153424)

• Purpose: Retroviral (MSCV) expression of tdTomato fluorescent protein
• Depositing Lab: Maki Nakayama
• Publication: Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 10.3389/fimmu.2020.00633. eCollection 2020.

Backbone

• Vector backbone: pMIII-Neo
• Backbone manufacturer: Maki Nakayama
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tdTomato
• Species: Other

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: 5pMIG
• 3′ sequencing primer: 3pMIG3

Resource Information

• Supplemental Documents:
  • 153424: pMIII-Neo-tdTomato
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Second gene is neomycin-resistant gene