A2_uSFFV
(Plasmid
#153415)
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PurposeExpresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUS
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Backbone manufacturerMaki Nakayama
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameA*02:01-P2A-human beta2 microglobulin
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SpeciesH. sapiens (human)
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Insert Size (bp)1512
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Entrez GeneB2M (a.k.a. IMD43)
- Promoter SFFV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
A2_uSFFV was a gift from Maki Nakayama (Addgene plasmid # 153415 ; http://n2t.net/addgene:153415 ; RRID:Addgene_153415) -
For your References section:
Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation Reporter-Based Detection System. Mann SE, Zhou Z, Landry LG, Anderson AM, Alkanani AK, Fischer J, Peakman M, Mallone R, Campbell K, Michels AW, Nakayama M. Front Immunol. 2020 Apr 9;11:633. doi: 10.3389/fimmu.2020.00633. eCollection 2020. 10.3389/fimmu.2020.00633 PubMed 32328071
Map uploaded by the depositor.
