HPV16 E7 TCR
PurposeExpress HPV16 E7 TCR
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||122728||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5600
- Total vector size (bp) 7400
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Growth instructionsTop10 cells may also be used.
Copy numberLow Copy
Gene/Insert nameHPV16 E7 TCR
Insert Size (bp)1800
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HPV16 E7 TCR was a gift from Christian Hinrichs (Addgene plasmid # 122728 ; http://n2t.net/addgene:122728 ; RRID:Addgene_122728)
For your References section:Engineered T cells targeting E7 mediate regression of human papillomavirus cancers in a murine model. Jin BY, Campbell TE, Draper LM, Stevanovic S, Weissbrich B, Yu Z, Restifo NP, Rosenberg SA, Trimble CL, Hinrichs CS. JCI Insight. 2018 Apr 19;3(8). pii: 99488. doi: 10.1172/jci.insight.99488. 10.1172/jci.insight.99488 PubMed 29669936