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pLTR-RD114A
(Plasmid #17576)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17576 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1 (Clontech)
  • Backbone size w/o insert (bp) 3417
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RD114 envelope glycoprotein
  • Alt name
    hybrid RD114 Env with cytoplasmic tail domain derived from the MLV 4070A Env
  • Species
    RD114 retrovirus
  • Insert Size (bp)
    2146
  • GenBank ID
    NC_009889

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Yasu Takeuchi
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use packaging construct encoding Tat

see Shimode et al. Virus Genes 45, 393-397 (2012) for details regarding discrepancies in accession number X87829. See Accession number NC_009889.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLTR-RD114A was a gift from Jakob Reiser (Addgene plasmid # 17576 ; http://n2t.net/addgene:17576 ; RRID:Addgene_17576)
  • For your References section:

    Transduction of bone-marrow-derived mesenchymal stem cells by using lentivirus vectors pseudotyped with modified RD114 envelope glycoproteins. Zhang XY, La Russa VF, Reiser J. J Virol. 2004 Feb . 78(3):1219-29. 10.1128/JVI.78.3.1219-1229.2004 PubMed 14722277