NFAT luciferase reporter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10959||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameIL-2 promoter
SpeciesH. sapiens (human)
Insert Size (bp)90
Entrez GeneIL2 (a.k.a. IL-2, TCGF, lymphokine)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer LucNRev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
A luciferase reporter containing three tandem repeats of a 30-bp fragment of the IL-2 promoter for analysis of NFAT activity. 30-bp fragment is GGAGG AAAAA CTGTT TCATA CAGAA GGCGT.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:NFAT luciferase reporter was a gift from Toren Finkel (Addgene plasmid # 10959 ; http://n2t.net/addgene:10959 ; RRID:Addgene_10959)
For your References section:Ras regulates NFAT3 activity in cardiac myocytes. Ichida M, Finkel T. J Biol Chem. 2001 Feb 2. 276(5):3524-30. 10.1074/jbc.M004275200 PubMed 11044444