nLuc-PTHR1
(Plasmid
#162577)
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PurposeExpresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells
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Depositing Lab
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Depositing OrganizationUniversity of Wisconsin, Madison
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162577 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 162577-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 162577-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 7700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameParathyroid Hormone Receptor 1
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Alt namePTHR1
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Alt namePTH1R
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Alt nameParathyroid hormone 1 receptor
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SpeciesH. sapiens (human)
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Insert Size (bp)2295
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GenBank IDAY449732.1
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Entrez GenePTH1R (a.k.a. EKNS, PFE, PTHR, PTHR1)
- Promoter CMV
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Tag
/ Fusion Protein
- Nanoluc (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV
- 3′ sequencing primer BGH
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPTHR1 in pcDNA3.1 was acquired from the cDNA Resource Center.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.07.25.500850 for bioRxiv preprint.
DNA (Catalog # 162577-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 162577-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
nLuc-PTHR1 was a gift from Sam Gellman (Addgene plasmid # 162577 ; http://n2t.net/addgene:162577 ; RRID:Addgene_162577) -
For your References section:
Kinetic and Thermodynamic Insights into Agonist Interactions with the Parathyroid Hormone Receptor-1 from a New NanoBRET Assay. Yu Z, Cary BP, Kim TW, Nguyen KD, Gardella TJ, Gellman SH. ACS Chem Biol. 2022 Nov 18;17(11):3148-3158. doi: 10.1021/acschembio.2c00595. Epub 2022 Oct 25. 10.1021/acschembio.2c00595 PubMed 36282520
