Gluc-LHCGR
(Plasmid
#221781)
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PurposeMeasurement of glycoprotein-glycoprotein receptor binding using the BRET phenomenon and measurement of membrane expression of the Gluc-LHCGR
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Depositing Lab
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Depositing OrganizationMedical University of Lublin
Located in Poland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221781 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 221781-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 221781-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV-LHCGR
- Total vector size (bp) 6658
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLHCGR
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Alt nameLuteinizing Hormone/Choriogonadotropin Receptor
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SpeciesH. sapiens (human)
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Insert Size (bp)2022
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Entrez GeneLHCGR (a.k.a. HHG, LCGR, LGR2, LH/CG-R, LH/CGR, LHR, LHRHR, LSH-R, ULG5)
- Promoter CMV
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Tags
/ Fusion Proteins
- Gaussia Luciferase (N terminal on insert)
- HA (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AGCTCGTTTAGTGAACCGTCAGATC
- 3′ sequencing primer CACACCACAGAAGTAAGGTTCC
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 221781-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 221781-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Gluc-LHCGR was a gift from Adolfo Rivero-Muller (Addgene plasmid # 221781 ; http://n2t.net/addgene:221781 ; RRID:Addgene_221781) -
For your References section:
Glycoprotein-glycoprotein Receptor Binding Detection Using Bioluminescence Resonance Energy Transfer. Adamczuk K, Ngo TH, Czapinski J, Rivero-Muller A. Endocrinology. 2024 Apr 29;165(6):bqae052. doi: 10.1210/endocr/bqae052. 10.1210/endocr/bqae052 PubMed 38679471
Map uploaded by the depositor.
