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murine TCR OTII-2A.pMIG II
(Plasmid #52112)


Item Catalog # Description Quantity Price (USD)
Plasmid 52112 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pMIG II
  • Backbone manufacturer
    Dario Vignali, Addgene plasmid 52107
  • Total vector size (bp) 8155
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    T cell receptor alpha chain
  • Alt name
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Tcra (a.k.a. Tcralpha)
  • Tag / Fusion Protein
    • P2A (from porcine teschovirus-1) (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    T cell receptor beta chain
  • Alt name
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Tcrb (a.k.a. TCRbeta, Tib)

Cloning Information for Gene/Insert 2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The cDNAs encoding the TCRa (lacking the stop codon) and TCRb chains were linked via a 2A peptide into a single open reading frame by recombinant PCR and cloned into a mouse stem cell virus (MSCV)-based retroviral vector containing an IRES-GFP cassette to facilitate the identification of marked cells.
The 2A peptide remains attached to the TCRa carboxy terminus and can be used to verify the complete separation of the two chains. Inclusion of a Gly-Ser-Gly (GSG) spacer between TCRa and the 2A peptide ensures complete ‘cleavage’. We have not seen any deleterious effects of the 2A peptide on the function of any proteins examined thus far.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    murine TCR OTII-2A.pMIG II was a gift from Dario Vignali (Addgene plasmid # 52112 ; ; RRID:Addgene_52112)
  • For your References section:

    Generation of T-cell receptor retrogenic mice. Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA. Nat Protoc. 2006;1(1):406-17. 10.1038/nprot.2006.61 PubMed 17406263