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Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II
(Plasmid #52092)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52092 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMIG II
  • Backbone manufacturer
    Dario Vignali, Addgene plasmid 52107
  • Total vector size (bp) 8155
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CD3 delta
  • Alt name
    CD3 antigen, delta polypeptide
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cd3d (a.k.a. T3d)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    CDC gamma
  • Alt name
    Cd3e CD3 antigen, gamma polypeptide
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cd3g (a.k.a. Ctg-3, Ctg3, T3g)
  • Tag / Fusion Protein
    • F2A (N terminal on insert)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    CD3 epsilon
  • Alt name
    Cd3e CD3 antigen, epsilon polypeptide
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cd3e (a.k.a. AI504783, CD3, CD3epsilon, T3e)
  • Tag / Fusion Protein
    • T2A (N terminal on insert)

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    CD3 zeta
  • Alt name
    Cd247
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cd247 (a.k.a. 4930549J05Rik, A430104F18Rik, AW552088, Cd3, Cd3-eta, Cd3-zeta, Cd3h, Cd3z, Cd3zeta, T3z, Tcrk, Tcrz)
  • Tags / Fusion Proteins
    • P2A (N terminal on insert)
    • IRES-EGFP (C terminal on backbone)

Cloning Information for Gene/Insert 4

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II was a gift from Dario Vignali (Addgene plasmid # 52092 ; http://n2t.net/addgene:52092 ; RRID:Addgene_52092)
  • For your References section:

    Scalable signaling mediated by T cell antigen receptor-CD3 ITAMs ensures effective negative selection and prevents autoimmunity. Holst J, Wang H, Eder KD, Workman CJ, Boyd KL, Baquet Z, Singh H, Forbes K, Chruscinski A, Smeyne R, van Oers NS, Utz PJ, Vignali DA. Nat Immunol. 2008 Jun;9(6):658-66. doi: 10.1038/ni.1611. Epub 2008 May 11. 10.1038/ni.1611 PubMed 18469818