Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

p1.2-Hyg-Fur
(Plasmid #162774)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 162774 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p1.1
  • Backbone manufacturer
    LMBT, LTD
  • Backbone size w/o insert (bp) 11914
  • Modifications to backbone
    The region containing the EMCV IRES and the DHFR ORF from the p1.1 expression vector is removed. Antibiotic resistance genes and the SV40 promoter and terminator regions are added.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human PACE/furin protease soluble
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2166
  • GenBank ID
    NM_002569
  • Entrez Gene
    FURIN (a.k.a. FUR, PACE, PCSK3, SPC1)
  • Promoter CHO EEF1A1 (Translation Elongation Factor 1 Alpha 1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AbsI (not destroyed)
  • 3′ cloning site XbaI/NheI (destroyed during cloning)
  • 5′ sequencing primer SQ-5CH6-F GCCGCTGCTTCCTGTGAC
  • 3′ sequencing primer SQ-3CH1-R ACAAACAGTTCTGAGACCG
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Soluble human PACE/furin ORF was obtained by PCR using plasmid SC118550 (Origene, USA) as a template, the ORF was brought in line with the reference sequence NM_002569 by adding six missing nucleotides (coding VQ).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p1.2-Hyg-Fur was a gift from Ivan Vorobiev (Addgene plasmid # 162774 ; http://n2t.net/addgene:162774 ; RRID:Addgene_162774)
  • For your References section:

    A Highly Productive CHO Cell Line Secreting Human Blood Clotting Factor IX. Kovnir SV, Orlova NA, Shakhparonov MI, Skryabin KG, Gabibov AG, Vorobiev II. Acta Naturae. 2018 Jan-Mar;10(1):51-65. PubMed 29713519