p1.2-Hyg-Fur
(Plasmid #162774)

• Purpose: Soluble human PACE/furin protease expression
• Depositing Lab: Ivan Vorobiev
• Depositing Organization: Federal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences
  Located in the Russian Federation
• Publication: Kovnir et al Acta Naturae. 2018 Jan-Mar;10(1):51-65.

Backbone

• Vector backbone: p1.1
• Backbone manufacturer: LMBT, LTD
• Backbone size w/o insert (bp): 11914
• Modifications to backbone: The region containing the EMCV IRES and the DHFR ORF from the p1.1 expression vector is removed. Antibiotic resistance genes and the SV40 promoter and terminator regions are added.
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human PACE/furin protease soluble
• Species: H. sapiens (human)
• Insert Size (bp): 2166
• GenBank ID: NM_002569
• Entrez Gene: FURIN (a.k.a. FUR, PACE, PCSK3, SPC1)
• Promoter: CHO EEF1A1 (Translation Elongation Factor 1 Alpha 1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AbsI (not destroyed)
• 3′ cloning site: XbaI/NheI (destroyed during cloning)
• 5′ sequencing primer: SQ-5CH6-F GCCGCTGCTTCCTGTGAC
• 3′ sequencing primer: SQ-3CH1-R ACAAACAGTTCTGAGACCG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Soluble human PACE/furin ORF was obtained by PCR using plasmid SC118550 (Origene, USA) as a template, the ORF was brought in line with the reference sequence NM_002569 by adding six missing nucleotides (coding VQ).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 162774-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 162774-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.