pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)
(Plasmid
#163074)
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PurposeEntry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)
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Depositing Lab
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Depositing OrganizationHarvard University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 163074-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 163074-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepENTR
- Backbone size w/o insert (bp) 2586
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Vector typeGateway Cloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGrx1-roGFP1-iEER
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SpeciesSynthetic
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Insert Size (bp)1275
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F(-21)
- 3′ sequencing primer M13R
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJulia Birk and Christian Appenzeller-Herzog
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see PMID: 23424194 for original reporter gene characterization.
DNA (Catalog # 163074-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 163074-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum) was a gift from Adam Cohen (Addgene plasmid # 163074 ; http://n2t.net/addgene:163074 ; RRID:Addgene_163074) -
For your References section:
Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology. Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE. Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5. 10.1038/s41467-020-17607-5 PubMed 32753572
