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xfz3N/pCS2+
(Plasmid #16328)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16328 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 16328-D50 50 μg of DNA in Tris buffer $495
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DNA 16328-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Vertebrate expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    frizzled-3
  • Alt name
    fz3
  • Alt name
    Nxfz3
  • Alt name
    N3
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    700
  • Mutation
    Extracellular ligand binding domain of Xenopus frizzled-3.
  • Entrez Gene
    fzd3.L (a.k.a. XELAEV_18028255mg, frizzled3, frz-3, frz3, fz3, fzd3)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct contains the amino terminus of xfz3, which includes the signal sequence, cysteine-rich domain (CRD) and the linker region. It terminates just before the first transmembrane region.
For sense transcripts, cut with NotI and transcribe with SP6.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    xfz3N/pCS2+ was a gift from Peter Klein (Addgene plasmid # 16328 ; http://n2t.net/addgene:16328 ; RRID:Addgene_16328)

  • For your References section:

    A role for frizzled 3 in neural crest development. Deardorff MA, Tan C, Saint-Jeannet JP, Klein PS. Development. 2001 Oct . 128(19):3655-63. PubMed 11585792
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