pAAV-hGfap-R-CaMP1.07-WPRE-SV40
(Plasmid #164140)

• Purpose: AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.
• Depositing Labs: Hirokazu Hirai, Hajime Hirase
• Publication: Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi: 10.1038/s41467-020-14378-x.

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Backbone

• Vector backbone: pAAV-hGFAP-*-WPRE-SV40
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: R-CaMP1.07
• Species: Synthetic
• Insert Size (bp): 1461
• Promoter: hGFAP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Prof Junichi Nakai (Tohoku University, Japan) who has constructed R-CaMP1.07.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: The 5’ ITR and cPPT/CTS has been removed from unknown reasons. However, high-titer AAV could be derived using this plasmid in the depositing lab.

This plasmid was made by Dr. Ayumu Konno at Gunma University (Hirai Lab) via collaboration.