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Cdc42 T17N sp64T
(Plasmid #16672)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16672 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 16672-D50 50 μg of DNA in Tris buffer $495
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DNA 16672-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pSP64T
  • Backbone manufacturer
    Available at Addgene (#15030)
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cdc42 T17N
  • Alt name
    Cdc42
  • Species
    H. sapiens (human)
  • Mutation
    T17N. Dominant negative.
  • Entrez Gene
    CDC42 (a.k.a. CDC42Hs, G25K, TKS)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This cDNA encodes a dominant negative version of Cdc42, a Rho GTPase.

The vector is pSP64T, which allows in vitro transcription from the SP6 promoter.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cdc42 T17N sp64T was a gift from De-Li Shi (Addgene plasmid # 16672 ; http://n2t.net/addgene:16672 ; RRID:Addgene_16672)

  • For your References section:

    Role of frizzled 7 in the regulation of convergent extension movements during gastrulation in Xenopus laevis. Djiane A, Riou J, Umbhauer M, Boucaut J, Shi D. Development. 2000 Jul . 127(14):3091-100. PubMed 10862746
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