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Addgene

ZP30 Fgf8
(Plasmid #16912)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16912 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fgf8
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    fgf8a (a.k.a. ac, ace, acere, cb110, etID309886.13, fgf-8, fgf8, id:ibd50, id:ibd5031, wu:fb73a0, wu:fb73a06)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fgf8 insert (XhoI-ApaI) from fgf8-CS2 cloned into XhoI-ApaI cut pSK+.

For antisense probe digest with XhoI, transcribe with T7 polymerase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZP30 Fgf8 was a gift from Randall Moon (Addgene plasmid # 16912 ; http://n2t.net/addgene:16912 ; RRID:Addgene_16912)
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