|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17056||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeZebrafish Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert namepax b
SpeciesD. rerio (zebrafish)
Insert Size (bp)1180
Entrez Genepax2a (a.k.a. PAXZF-B, Pax-2, cb378, noi, pax-b, pax2.1, pax2a1, pax[zf-b], paxb)
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl II (unknown if destroyed)
- 3′ cloning site BglII (unknown if destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Insert: pax b sense or antisense orientation.
Insert size: 1.18 Kb.
Transcription: linearize at EcoR1, transcribe with SP6.
Constructed by Greg Kelly on date of 9/21/92, page 118 Book 1.
Sepecies of Sequence Origin: Zebrafish.
Note for Use:
This construct is used to make DIG probes or for making RNA suitable for injections.
Citation: Kelly and Moon, Developmental Genetics (1995)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZE7 Pax2 was a gift from Randall Moon (Addgene plasmid # 17056 ; http://n2t.net/addgene:17056 ; RRID:Addgene_17056)
Map uploaded by the depositor.