ZE7 Pax2
(Plasmid #17056)

• Depositing Lab: Randall Moon
• Publication: Zebrafish Expression Plasmids (unpublished)

Backbone

• Vector backbone: SP64T
  (Search Vector Database)
• Backbone size w/o insert (bp): 3000
• Vector type: Zebrafish Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pax b
• Alt name: pax2
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1180
• Entrez Gene: pax2a (a.k.a. PAXZF-B, Pax-2, cb378, noi, pax-b, pax2.1, pax2a1, pax[zf-b], paxb)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II (unknown if destroyed)
• 3′ cloning site: BglII (unknown if destroyed)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: pax b sense or antisense orientation.
Insert size: 1.18 Kb.
Transcription: linearize at EcoR1, transcribe with SP6.

Plasmid History:
Constructed by Greg Kelly on date of 9/21/92, page 118 Book 1.
Sepecies of Sequence Origin: Zebrafish.

Note for Use:
This construct is used to make DIG probes or for making RNA suitable for injections.
Citation: Kelly and Moon, Developmental Genetics (1995)