lentiCRISPRi(v1)-Blast
(Plasmid
#170067)
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PurposeExpresses dCas9 repressor KRAB-dCas9 and blasticidin resistance
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170067 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFUGW
- Backbone size w/o insert (bp) 8800
- Total vector size (bp) 13100
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKRAB-dCas9
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Insert Size (bp)4300
- Promoter EFS-NS
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Tag
/ Fusion Protein
- 2A and 2xHA tag
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EFS-NS-fwd (5'-gagaaccgtatataagtgcagt-3')
- 3′ sequencing primer blast-R (5'-gctctttcaatgagggtgga-3') (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.04.07.438882 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
lentiCRISPRi(v1)-Blast was a gift from Neville Sanjana (Addgene plasmid # 170067 ; http://n2t.net/addgene:170067 ; RRID:Addgene_170067) -
For your References section:
Discovery of target genes and pathways at GWAS loci by pooled single-cell CRISPR screens. Morris JA, Caragine C, Daniloski Z, Domingo J, Barry T, Lu L, Davis K, Ziosi M, Glinos DA, Hao S, Mimitou EP, Smibert P, Roeder K, Katsevich E, Lappalainen T, Sanjana NE. Science. 2023 May 19;380(6646):eadh7699. doi: 10.1126/science.adh7699. Epub 2023 May 19. 10.1126/science.adh7699 PubMed 37141313
Map uploaded by the depositor.
