lacI-Ptac-lacO-BsaI-BsaI-IMBB4-pUC
(Plasmid #170772)

• Purpose: test plasmid for Golden Gate assembly, expresses lacI repressor
• Depositing Lab: Ichiro Matsumura
• Publication: Matsumura et al Biotechniques. 2022 May;72(5):185-193. doi: 10.2144/btn-2021-0083. Epub 2022 Mar 8.

Backbone

• Vector backbone: pUC
• Backbone manufacturer: pTwist
• Backbone size w/o insert (bp): 3
• Total vector size (bp): 3760
• Modifications to backbone: encodes promoter, ribosome binding site, lacI structural gene, tac promoter, lac operon, BsaI-EcoRV-BsaI multiple cloning site

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: lacI
• Species: Synthetic
• Insert Size (bp): 1082
• Promoter: lacI promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MfeI (not destroyed)
• 3′ cloning site: NsiI (not destroyed)
• 5′ sequencing primer: part sequenced in different plasmid prior to subcloning
• 3′ sequencing primer: part sequenced in different plasmid prior to subcloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was designed to test Golden Gate assembly protocols