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Golden Gate assembly of BioBrick-compliant parts using Type II restriction endonucleases.
Matsumura I
Biotechniques. 2022 May;72(5):185-193. doi: 10.2144/btn-2021-0083. Epub 2022 Mar 8. PubMed Article

Plasmids from Article

ID Plasmid Purpose
170767Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadAMethylase expression vector for 5RM assembly: expresses M.XbaI, M.EcoRI and M.SalI DNA methyltransferases in E. coli
170768Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadAMethylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coli
170769tagRFP-IMBB4-pUCtest plasmid for 5RM assembly, encodes fluorescent red protein
170770lacI-Ptac-lacO-IMBB4-pUCtest plasmid for 5RM assembly, expresses lacI repressor
170771BsaI-tagRFP-BsaI-pUC-kantest plasmid for Golden Gate assembly, encodes fluorescent red protein
170772lacI-Ptac-lacO-BsaI-BsaI-IMBB4-pUCtest plasmid for Golden Gate assembly, expresses lacI repressor

Antibodies from Article