BsaI-tagRFP-BsaI-pUC-kan
(Plasmid
#170771)
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Purposetest plasmid for Golden Gate assembly, encodes fluorescent red protein
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170771 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC
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Backbone manufacturerGenScript
- Backbone size w/o insert (bp) 2579
- Total vector size (bp) 2730
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametagRFP
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SpeciesSynthetic
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Insert Size (bp)695
- Promoter none
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer part sequenced by manufacturer (GenScript)
- 3′ sequencing primer part sequenced by manufacturer (GenScript) (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe tagRFP gene was synthesized, cloned and sequenced by GenScript.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was designed to test Golden Gate assembly protocols
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BsaI-tagRFP-BsaI-pUC-kan was a gift from Ichiro Matsumura (Addgene plasmid # 170771 ; http://n2t.net/addgene:170771 ; RRID:Addgene_170771) -
For your References section:
Golden Gate assembly of BioBrick-compliant parts using Type II restriction endonucleases. Matsumura I. Biotechniques. 2022 May;72(5):185-193. doi: 10.2144/btn-2021-0083. Epub 2022 Mar 8. 10.2144/btn-2021-0083 PubMed 35255734
Map uploaded by the depositor.
