|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||39860||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemonomeric streptavidin
Alt namestreptavidin rhizavidin hybrid
Insert Size (bp)402
- Promoter T7
/ Fusion Proteins
- 6xHis (N terminal on backbone)
- FLAG (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 Promoter (Common Sequencing Primers)
Please note the YPet fluorescent protein present in the vector backbone is not expressed with the streptavidin insert.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRSET-mSA was a gift from Sheldon Park (Addgene plasmid # 39860 ; http://n2t.net/addgene:39860 ; RRID:Addgene_39860)
For your References section:Stable, high-affinity streptavidin monomer for protein labeling and monovalent biotin detection. Lim KH, Huang H, Pralle A, Park S. Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. Epub 2012 Aug 8. 10.1002/bit.24605 PubMed 22806584