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pET21a-Traptavidin
(Plasmid #24753)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24753 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Traptavidin
  • Species
    Streptomyces avidinii
  • Insert Size (bp)
    405
  • Mutation
    Streptavidin with 2 amino acid mutations: S52G and R53D
  • GenBank ID
    GU952124
  • Tag / Fusion Protein
    • His-6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Traptavidin has a lower off-rate and lower on-rate for biotin conjugates, as well as higher mechanical strength and higher thermal stability. It is tetramer, refolded from inclusion bodies.

This particular material is subject to a UK priority patent application (0919102.4) filed on 30th October 2009

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a-Traptavidin was a gift from Mark Howarth (Addgene plasmid # 24753)
  • For your References section:

    A streptavidin variant with slower biotin dissociation and increased mechanostability. Chivers CE, Crozat E, Chu C, Moy VT, Sherratt DJ, Howarth M. Nat Methods. 2010 Apr 11. ():. 10.1038/nmeth.1450 PubMed 20383133