PurposeDead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20859||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5443
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)411
MutationTriple mutant of wt Streptavidin N23A,S27D,S45A
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Please email [email protected] with intended use and/or targets for this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET21a-Streptavidin-Dead was a gift from Alice Ting (Addgene plasmid # 20859 ; http://n2t.net/addgene:20859 ; RRID:Addgene_20859)
For your References section:A monovalent streptavidin with a single femtomolar biotin binding site. Howarth M, Chinnapen DJ, Gerrow K, Dorrestein PC, Grandy MR, Kelleher NL, El-Husseini A, Ting AY. Nat Methods. 2006 Apr . 3(4):267-73. 10.1038/nmeth861 PubMed 16554831