|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20857||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5443
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)963
/ Fusion Protein
- His (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Please email [email protected] with your intended use and/or targets for this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET21a-BirA was a gift from Alice Ting (Addgene plasmid # 20857 ; http://n2t.net/addgene:20857 ; RRID:Addgene_20857)
For your References section:Targeting quantum dots to surface proteins in living cells with biotin ligase. Howarth M, Takao K, Hayashi Y, Ting AY. Proc Natl Acad Sci U S A. 2005 May 24. 102(21):7583-8. 10.1073/pnas.0503125102 PubMed 15897449