|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20856||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5300
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1005
MutationC-terminal ER targeting sequence (KDEL)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Please email [email protected] with the intended use and/or targets for this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDisplay-BirA-ER was a gift from Alice Ting (Addgene plasmid # 20856 ; http://n2t.net/addgene:20856 ; RRID:Addgene_20856)
For your References section:Monovalent, reduced-size quantum dots for imaging receptors on living cells. Howarth M, Liu W, Puthenveetil S, Zheng Y, Marshall LF, Schmidt MM, Wittrup KD, Bawendi MG, Ting AY. Nat Methods. 2008 May . 5(5):397-9. 10.1038/nmeth.1206 PubMed 18425138