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PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 36047 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | ||
Cloning Grade DNA | 36047-DNA.cg | 2 µg of cloning grade DNA in Tris buffer | 1 | $95 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5333
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Modifications to backboneReplaces MCS
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBirA(R118G)-HA
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SpeciesE. coli
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Insert Size (bp)993
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MutationR118G (highly promiscuous form)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
Information for Cloning Grade DNA (Catalog # 36047-DNA.cg) ( Back to top )
Purpose
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
Delivery
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
Quality Control
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 MCS-BirA(R118G)-HA was a gift from Kyle Roux (Addgene plasmid # 36047 ; http://n2t.net/addgene:36047 ; RRID:Addgene_36047) -
For your References section:
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. Roux KJ, Kim DI, Raida M, Burke B. J Cell Biol. 2012 Mar 12. 10.1083/jcb.201112098 PubMed 22412018