BirA(R118G)-HA destination vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53581||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1 MCS-BirA(R118G)-HA
Backbone manufacturerKyle Roux, Addgene Plasmid 36047
Modifications to backbonepcDNA3.1 MCS-BirA(R118G)-HA was cut with HpaI and ligated to a blunt ended selection cassette (Gateway conversion kit, cassette B) containing AttR sites flanking the ccdB gene and the chloramphenicol resistance gene.
Vector typeMammalian Expression
- Promoter CMV
Selectable markersNeomycin (select with G418)
/ Fusion Proteins
- BirA(R118G) (C terminal on insert)
- HA tag (C terminal on insert)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BirA(R118G)-HA destination vector was a gift from Karl Kramer (Addgene plasmid # 53581 ; http://n2t.net/addgene:53581 ; RRID:Addgene_53581)