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Purpose(Empty Backbone) To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 74223 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | ||
| Cloning Grade DNA | 74223-DNA.cg | 2 µg of cloning grade DNA in Tris buffer | 1 | $95 | ||
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA 3.1
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Backbone manufacturerThermo Fisher Scientific
- Backbone size (bp) 6147
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersNeomycin (select with G418)
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Tags
/ Fusion Proteins
- Myc (N terminal on backbone)
- humanized A. aeolicus BioID2 with R40G (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
Information for Cloning Grade DNA (Catalog # 74223-DNA.cg) ( Back to top )
Purpose
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
Delivery
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
Quality Control
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
myc-BioID2-MCS was a gift from Kyle Roux (Addgene plasmid # 74223 ; http://n2t.net/addgene:74223 ; RRID:Addgene_74223) -
For your References section:
An improved smaller biotin ligase for BioID proximity labeling. Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844. 10.1091/mbc.E15-12-0844 PubMed 26912792
Map uploaded by the depositor.
