Anti-c-Myc [9E10] - Chimeric
(Antibody #194520)

• Purpose: Anti-c-Myc chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a chicken IgY Fc.
• Depositing Lab: Melina Fan
• Publication: Evan et al Mol Cell Biol. 1985 Dec;5(12):3610-6. doi: 10.1128/mcb.5.12.3610-3616.1985.
  
• Recommended Applications: Western Blot

Features

• Source Species: G. gallus (chicken)
• Isotype: IgY
• Light Chain Type: kappa
• Amino Acid Sequences:
  Sequences (2) — Accept Affinity Reagent Sequence Policy

Production & Usage

• Antigen Plasmid: pAAV.CAG.GFPsm-myc.WPRE.SV40
• Purification: Purification from cell culture supernatant using affinity chromatography followed by buffer exchange with centrifugal columns.
• Storage Buffer: PBS + 1 mM sodium azide

Delivery

• Concentration: 0.9–1.1 mg/mL
• Storage: Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
• Shipment: Shipped on blue ice.

Resource Information

• Persistent Resource Identifier: RRID:AB_2928077

Terms and Licenses

• Academic/Nonprofit Terms:
  • Research Use Only Terms
• Industry Terms:
  • Research Use Only Terms

Target Antigen

Protein	Myc proto-oncogene protein
Antigen Description	Amino acids 408–432 of human Myc
Antigen Amino Acid Sequence	AEEQKLISEEDLLRKRREQLKHKLE View Entire Sequence
Species	H. sapiens (human)
Gene	MYC
Alternative Names	Class E basic helix-loop-helix protein 39 (bHLHe39) Proto-oncogene c-Myc Transcription factor p64
External References	Uniprot ID: P01106

Applications (1)

Assay

Western Blot

Sample Species

H. sapiens (human)

Result

Pass

Information

Application Information

×

Quality Control

Each lot of antibody undergoes testing, either in-house or through outside labs, to confirm recognition of the expected target or to confirm expected labeling across a tissue panel in one of the antibody’s recommended applications. The specific experiments performed varies for each antibody catalog item. Whenever possible, the experiment is performed in parallel with a previous lot of recombinant antibody or the hybridoma-derived equivalent to ensure potency and specificity have been retained.

Learn more: Antibody Production

Recommended Applications

These recommendations are based on a thorough review of experiments performed at Addgene or at outside labs. Addgene has partnered with outside labs in order to provide careful testing of our antibodies in applications that we are not able to test at our own facilities. Addgene works carefully with these trusted partner labs to ensure that experiments include proper controls and sufficient experimental details and these labs are denoted below with an “Addgene Partner” badge. Please note that we show results for applications that work well with our antibody (Recommended) as well as those that do not (Not recommended) so be sure to check the result!

Antibody Type

Many of Addgene’s recombinant antibodies encode sequences that were originally derived from hybridoma lines. In many cases, we provide results from assays performed with this hybridoma-derived antibody in addition to results from assays performed with the recombinant antibody. To prevent confusion, the application data represented on Addgene’s website clearly notes whether the hybridoma material or recombinant antibody material were used in the assay. In some cases, Recommended Applications are based on results from experiments performed with a hybridoma-derived antibody. If you have any trouble with the recombinant antibody for this same application please contact us at

Western Blot

Recommended

1 image

Lab: : Addgene

Antibody Type : Recombinant

Sample Species : H. sapiens (human)

Result : Pass

View Details

Image 1 (of 1): HEK293 cells were left untransfected or transfected with pAAV.CAG.GFPsm-myc.WPRE.SV40 (+98926). Whole cell lysates were prepared and probed with Anti-Myc [9e10] and anti-chicken IgY secondary antibody. The blot was stripped and reprobed with anti-vinculin as a loading control.
Credit: Addgene

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Anti-c-Myc [9E10] - Chimeric

(Antibody #194520)

Western Blot

Lab:: Addgene

Antibody Type

Recombinant

Antibody Concentration

1 µg/mL

Sample Species

H. sapiens (human)

Sample Type

HEK293

Result

Pass

Details

30 µg total HEK293 cell lysate was reduced and separated by denaturing SDS-PAGE

Blocking conditions: 5% non-fat dry milk in TBST pH 7.4, 45 min, room temperature

Primary antibody: 1 µg/mL in 5% non-fat dry milk in TBST pH 7.4, 45 min, room temperature

Secondary antibody: Goat anti-chicken IgY (H+L) HRP (Thermo Fisher, catalog number A16054) in 5% non-fat dry milk in TBST, 45 min, room temperature

Predicted molecular weight: 40 kDa

Addgene Western Blot protocol

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Addgene Comments

This antibody has been engineered from sequences from a mouse monoclonal parent antibody. By necessity, some mouse sequences are retained. When multiplexing with mouse antibodies, we recommend using Fc-directed, pre-adsorbed secondary antibodies.