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An improved smaller biotin ligase for BioID proximity labeling.
Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ
Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
74223myc-BioID2-MCSTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
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74224MCS-BioID2-HATo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
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80899MCS-13X Linker-BioID2-HATo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.
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80900mycBioID2-pBABE-puroTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); a small MCS is present downstream of myc tag and BioID2 tag
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92308mycBioID2-13X Linker-MCSTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.
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