Purpose(Empty Backbone) To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80899||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||80899-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
Backbone manufacturerThermo Fisher Scientific
- Backbone size (bp) 6335
Vector typeMammalian Expression
- Promoter CMV
Selectable markersNeomycin (select with G418)
/ Fusion Proteins
- humanized A. aeolicus BioID2 with R40G (C terminal on backbone)
- HA (C terminal on backbone)
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Please note that the location of the 13X Linker-BioID2-HA insert in this plasmid is at bp# 975-1907.
Information for Cloning Grade DNA (Catalog # 80899-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MCS-13X Linker-BioID2-HA was a gift from Kyle Roux (Addgene plasmid # 80899 ; http://n2t.net/addgene:80899 ; RRID:Addgene_80899)
For your References section:An improved smaller biotin ligase for BioID proximity labeling. Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844. 10.1091/mbc.E15-12-0844 PubMed 26912792