Purpose(Empty Backbone) To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80899||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerThermo Fisher Scientific
- Backbone size (bp) 6335
Vector typeMammalian Expression
- Promoter CMV
Selectable markersNeomycin (select with G418)
/ Fusion Proteins
- humanized A. aeolicus BioID2 with R40G (C terminal on backbone)
- HA (C terminal on backbone)
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Please note that the location of the 13X Linker-BioID2-HA insert in this plasmid is at bp# 975-1907.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MCS-13X Linker-BioID2-HA was a gift from Kyle Roux (Addgene plasmid # 80899 ; http://n2t.net/addgene:80899 ; RRID:Addgene_80899)
For your References section:An improved smaller biotin ligase for BioID proximity labeling. Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844. 10.1091/mbc.E15-12-0844 PubMed 26912792