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pDisplay-AP-CFP-TM
(Plasmid #20861)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20861 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP-CFP-TM
  • Species
    E. Coli
  • Insert Size (bp)
    1092
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • CFP (C terminal on insert)
    • myc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please email [email protected] with intended use and/or targets for this plasmid

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDisplay-AP-CFP-TM was a gift from Alice Ting (Addgene plasmid # 20861)
  • For your References section:

    Targeting quantum dots to surface proteins in living cells with biotin ligase. Howarth M, Takao K, Hayashi Y, Ting AY. Proc Natl Acad Sci U S A. 2005 May 24. 102(21):7583-8. 10.1073/pnas.0503125102 PubMed 15897449