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Anti-6xHis [N144/14] - Chimeric
(Antibody #196562)


Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 196562-rAb 100 µg of purified recombinant antibody $250 *
Recombinant Antibody trial size 196562-rAb.T 20 µg of purified recombinant antibody $85 *

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Production & Usage

  • Antigen Plasmid
  • Purification
    Purification from cell culture supernatant using affinity chromatography followed by buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide


  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Shipped at room temperature or on blue ice.

Resource Information

  • Persistent Resource Identifier

Terms and Licenses

Target Antigen

Protein 6x HIS
Antigen Description Identified as off-target mAb in screen for anti-ProtocadherinGammaA3 NeuroMabs (immunogen was 6xHis fusion protein amino acids 720–804, variable portion of cytoplasmic C-terminus, of mouse ProtocadherinGammaA3). Target identified as 6xHis (also known as Hexahistidine and Polyhistidine) by ELISA, immunofluorescence and immunoblot against other 6xHis-tagged fusion proteins.
Species Synthetic
Alternative Names
  • 6xHIS
  • 6x-HIS
  • His6
  • Hexahistidine

Applications (1)

Western Blot

1 image
Western Blot image by Addgene
Lab: Addgene
Antibody Type
Sample Species
H. sapiens (human)

Addgene Comments

This antibody has been engineered from sequences from a mouse monoclonal parent antibody. By necessity, some mouse sequences are retained. When multiplexing with mouse antibodies, we recommend using Fc-directed, pre-adsorbed secondary antibodies.
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-6xHis [N144/14] - Chimeric was a gift from Melina Fan, James Trimmer (Addgene antibody # 196562 ; ; RRID:AB_2928072)
  • For your References section:

    A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360