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BirA in pET28a (w400-2)
(Plasmid #26624)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26624 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5247
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10F', 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BirA
  • Alt name
    biotin ligase
  • Species
    E.coli
  • Insert Size (bp)
    967
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco I (destroyed during cloning)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

BirA gene was amplified from E.coli TOP10F' strain and subcloned into the pET28A vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BirA in pET28a (w400-2) was a gift from Eric Campeau (Addgene plasmid # 26624)