|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26054||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5443
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCore Traptavidin
Insert Size (bp)387
MutationStreptavidin with 2 amino acid mutations: S52G R53D. No His tag.
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Traptavidin has a lower off-rate and lower on-rate for biotin conjugates, as well as higher mechanical strength and higher thermal stability. It is a tetramer, refolded from inclusion bodies. This plasmid encodes 'core' traptavidin, which does not contain a His tag.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET21a-Core Traptavidin was a gift from Mark Howarth (Addgene plasmid # 26054 ; http://n2t.net/addgene:26054 ; RRID:Addgene_26054)
For your References section:A streptavidin variant with slower biotin dissociation and increased mechanostability. Chivers CE, Crozat E, Chu C, Moy VT, Sherratt DJ, Howarth M. Nat Methods. 2010 Apr 11. ():. 10.1038/nmeth.1450 PubMed 20383133