|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||171232||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression, Bacterial Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namehuman androgen receptor Ser650Ala mutant
SpeciesH. sapiens (human)
Insert Size (bp)3000
Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV_fwd_primer (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Mutation in an AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCIneo-AR-S650A was a gift from Steven Balk (Addgene plasmid # 171232 ; http://n2t.net/addgene:171232 ; RRID:Addgene_171232)