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pcDNA3.1(+)-C-6His-mEsrra
(Plasmid #173152)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173152 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1(+)-C-6His
  • Backbone manufacturer
    Genescript
  • Backbone size w/o insert (bp) 5432
  • Total vector size (bp) 6710
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ORF clone Estrogen-related receptor alpha (Esrra)
  • Alt name
    ERRa
  • Alt name
    Esrra
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1278
  • Mutation
    Wild type
  • GenBank ID
    NM_007953.2 NM_007953.2
  • Entrez Gene
    Esrra (a.k.a. ERRalpha, Err1, Estrra, Nr3b1)
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Custom generated by Genescript upon order.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.11.16.468891 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)-C-6His-mEsrra was a gift from Brijesh Kumar Singh (Addgene plasmid # 173152 ; http://n2t.net/addgene:173152 ; RRID:Addgene_173152)

  • For your References section:

    Esrra regulates Rplp1-mediated translation of lysosome proteins suppressed in metabolic dysfunction-associated steatohepatitis and reversed by alternate day fasting. Tripathi M, Gauthier K, Sandireddy R, Zhou J, Guptta P, Sakthivel S, Teo WW, Naing YT, Arul K, Tikno K, Park SH, Wu Y, Wang L, Bay BH, Sun L, Giguere V, Chow PKH, Ghosh S, McDonnell DP, Yen PM, Singh BK. Mol Metab. 2024 Sep;87:101997. doi: 10.1016/j.molmet.2024.101997. Epub 2024 Jul 19. 10.1016/j.molmet.2024.101997 PubMed 39032642
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