pcDNA4-TO-Myc-rZAP
(Plasmid #17381)

• Depositing Lab: Stephen Goff
• Publication: Gao et al Science. 2002 Sep 6. 297(5587):1703-6.

Backbone

• Vector backbone: pCDNA4 TO2 myc HisB
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZAP
• Alt name: rZAP
• Alt name: Zinc-finger Antiviral Protein
• Species: R. norvegicus (rat)
• Entrez Gene: Zc3hav1 (a.k.a. ARTD13, PARP13, Zap)
• Tag / Fusion Protein:
  • Myc (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The NZAP fragment was excised from pBabe-NZAP-Zeo with EcoRI-NotI and cloned into pCDNA4/TO2-myc-HisB to generate a myc-tagged
NZAP. The C-terminal portion of ZAP was cloned from a Rat2 cell cDNA library by PCR using sense primer CZAP-SP (5’-GAGCTCTCTGGGCTTAACC-3’) and antisense primer CZAP-AP (5’-
ATATAGGCGGCCGCCCTCTGGACCTCTTCTCTTC-3’). The sense primer lies
upstream from an internal NheI site in NZAP; the antisense primer introduces a NotI site immediately downstream from the coding sequence to facilitate its cloning into the myc-tagged expression vector.