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pcDNA3-DN-hCUL5-FLAG
(Plasmid #15823)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15823 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cullin5
  • Alt name
    Cullin 5
  • Alt name
    Cul5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Mutation
    Dominant negative: N441
  • Entrez Gene
    CUL5 (a.k.a. CUL-5, VACM-1, VACM1)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

C-terminal truncation of hCul5: amino acids 1-441.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-DN-hCUL5-FLAG was a gift from Wade Harper (Addgene plasmid # 15823 ; http://n2t.net/addgene:15823 ; RRID:Addgene_15823)
  • For your References section:

    Identification of substrates for F-box proteins. Jin J, Ang XL, Shirogane T, Wade Harper J. Methods Enzymol. 2005 . 399():287-309. 10.1016/S0076-6879(05)99020-4 PubMed 16338364