pcDNA HisB TO rFez1
(Plasmid
#17385)
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Depositing Lab
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Depositing OrganizationColumbia University
Located in the United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17385 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 17385-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 17385-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCDNA4 TO2 myc HisB
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFEZ1
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Alt nameFez1
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Alt namefasciculation and elongation protein zeta-1
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1200
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Entrez GeneFez1
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Tags
/ Fusion Proteins
- myc (C terminal on backbone)
- his (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full-length Rat2 FEZ1 sequences, 1179 bp, was amplified using cDNA from the wild-type Rat2 fibroblasts or the mutant R3-2 line as templates. The sequences of the PCR primers for generation of C-terminal myc-His-tagged FEZ1 were as follows: forward primer, FEZ1-S1-BamHI, 5'-GCTGATGGATCCACCATGGAGGCTC CACTGGTGAGT3'; reverse primer, FEZ1-A3-XhoI, 5'-GCAACGCTCGAGCAGGTTGGACAGAGCACTTTTAAA-3'.
DNA (Catalog # 17385-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 17385-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA HisB TO rFez1 was a gift from Stephen Goff (Addgene plasmid # 17385 ; http://n2t.net/addgene:17385 ; RRID:Addgene_17385) -
For your References section:
Overexpression of fasciculation and elongation protein zeta-1 (FEZ1) induces a post-entry block to retroviruses in cultured cells. Naghavi MH, Hatziioannou T, Gao G, Goff SP. Genes Dev. 2005 May 1. 19(9):1105-15. 10.1101/gad.1290005 PubMed 15879557