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pCDNA3-mitoT
(Plasmid #174519)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174519 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 174519-D50 50 μg of DNA in Tris buffer $495
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DNA 174519-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MitoT synthetic tether
  • Alt name
    MitoT
  • Species
    H. sapiens (human), Synthetic; E.coli
  • Insert Size (bp)
    468
  • Promoter CMV
  • Tags / Fusion Proteins
    • 6xHis (C terminal on insert)
    • eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer 5’-AAAGGATCCATGGCGATGCTGGTCCTAGTACCC-3’
  • 3′ sequencing primer 5’-CAAGAATTCGTGATGGTGATGGTGATGGAAGTAGATGCAGTACCCA-3’
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-mitoT was a gift from Oleh Khalimonchuk (Addgene plasmid # 174519 ; http://n2t.net/addgene:174519 ; RRID:Addgene_174519)

  • For your References section:

    Protease OMA1 modulates mitochondrial bioenergetics and ultrastructure through dynamic association with MICOS complex. Viana MP, Levytskyy RM, Anand R, Reichert AS, Khalimonchuk O. iScience. 2021 Jan 29;24(2):102119. doi: 10.1016/j.isci.2021.102119. eCollection 2021 Feb 19. 10.1016/j.isci.2021.102119 PubMed 33644718
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