mCherry-Fis1
(Plasmid
#182580)
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PurposeExpresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182580 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-Fis1
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Insert Size (bp)813
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Tag
/ Fusion Protein
- Fis1 (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-Fis1 was a gift from Uri Manor (Addgene plasmid # 182580 ; http://n2t.net/addgene:182580 ; RRID:Addgene_182580) -
For your References section:
Actin chromobody imaging reveals sub-organellar actin dynamics. Schiavon CR, Zhang T, Zhao B, Moore AS, Wales P, Andrade LR, Wu M, Sung TC, Dayn Y, Feng JW, Quintero OA, Shadel GS, Grosse R, Manor U. Nat Methods. 2020 Sep;17(9):917-921. doi: 10.1038/s41592-020-0926-5. Epub 2020 Aug 10. 10.1038/s41592-020-0926-5 PubMed 32778832