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Actin chromobody imaging reveals sub-organellar actin dynamics.
Schiavon CR, Zhang T, Zhao B, Moore AS, Wales P, Andrade LR, Wu M, Sung TC, Dayn Y, Feng JW, Quintero OA, Shadel GS, Grosse R, Manor U
Nat Methods. 2020 Sep;17(9):917-921. doi: 10.1038/s41592-020-0926-5. Epub 2020 Aug 10.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
182576LifeAct-GFP-Fis1Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence
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182577LifeAct-GFP-cytoB5RRExpresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequence
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182579mCherry-Cytb5Expresses mCherry targeted to the ER via the CytB5RR tail anchor sequence
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182580mCherry-Fis1Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence
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Antibodies from Article