pAAV.GFAP.iGluSnFR3.v857.GPI
(Plasmid #178338)

• Purpose: Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
• Depositing Lab: Kaspar Podgorski
• Depositing Organization: HHMI, Janelia Research Campus
  Located in the United States of America
• Publication: Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s41592-023-01863-6.
• Citations:

  1 Article

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• Serotype: Select serotype for details See details about
• Pricing: Select serotype and quantity $ USD for preparation of µL virus + $34 USD for plasmid.
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Backbone

• Vector backbone: pAAV GFAP
• Backbone size w/o insert (bp): 4836
• Total vector size (bp): 6624
• Vector type: Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: <8 hours
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pAAV GFAP iGluSnFR3 v857.GPI
• Alt name: iGluSnFR3 v857.GPI
• Alt name: iGluSnFR3 556dot857.GPI
• Alt name: v857.GPI
• Species: M. musculus (mouse), R. norvegicus (rat), Synthetic
• Insert Size (bp): 1788
• Tags / Fusion Proteins:
  • IgK-chain (N terminal on insert)
  • Myc epi tag (C terminal on insert)
  • GPI Anchor (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 178338-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 178338-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.